Abstract

OBJECTIVE

The aim of this study was to compare the reliability of hybridization and direct fluorescent antibody tests for diagnosis of genital Chlamydia trachomatis infections.

METHODS

Cervical specimens from 100 infertile women were evaluated with hybridizasyon, direct fluorescent antibody and cell culture method to be used as gold standard and sensitivity, specificity, positive and negative predictive values of the three methods were compared.

RESULTS

C. trachomatis was determined on 11 of the samples. Seven and 19 positive samples were found respectively through direct fluorescent antibody and hybridization tests. It was calculated %54,5 and %81,8 sensitivity, %98,9 and %88,8 specificity, %85,7 and %47,4 positive predictive value, %94,6 and %97,5 negative predictive value respectively in direct fluorescent antibody and hybridization tests.

CONCLUSION

It was concluded that a single method has not a precise sensitivity and specificity for C. trachomatis diagnosis and the results should be confirmed by other methods and the hybridization method is more rapid and effective than the other methods.